Immunolabeling

Tokuyasu method in cell cultures and tissues with criotalls

Inmunolocalization antigens in cell cultures and tissues. The samples are prepared using a mixture of chemical methods (fixation and cryoprotection) and physical methods (fixed in liquid nitrogen or propane). Subsequently, ultrathin cuts are made in cold conditions (-120ºC) on which the immunolocalization procedure (incubation in gold-labeled primary and secondary antibodies will take place in preparation for TEM observation.  

 Elena Garcia-Fruitós, IBB, Dpt de Genètica i Microbiologia, UAB.

Microscope used: JEOL JEM-1400

 

Immunolocalizations images with hydrophilic resins

Antigen immunolocalization of citoplasm and nucleus, as well as surface antigen immunolocalization of cell cultures and tissues. The samples are prepared using a mixture of chemical methods (fixation, cryoprotection, embedding in Lowicryl resin and UV) and physical methods(liquid nitrogen or propane fixation). Subsequently, ultrathin cuts are made at room temperature on which the immulocalization procedure (incubation in gold-labeled primary and secondary antibodies) will take place in preparation for TEM observation.   

 Carolina Rodríguez-Cariño, CreSA, UAB-IRTA.

 

 María Vicario, Institut de Recerca Hospital Universitari Vall d’Hebron i Alejandro Sánchez-Chardi, Servei de Microscòpia.

Microscope used: JEOL JEM-1400

 

 

 

Campus d'excel·lència internacional U A B