Assay request | Servei de Genòmica

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Requirements and conditions for the assays:

Schedule: The “Servei” is open from 8:30h to 16:30h, from Monday to Friday
Telephone: +34935868945/47

Shipping conditions: All batches of samples must have indicated date, number of tubes or plates, storage temperature and solvent. After the delivery of results, samples will be kept at the SG for one month.

Condiciones del envío de las muestras: Se debe indicar la fecha en que se realiza el envío, el número de tubos o placas que se envían, la temperatura y en que disolvente están.Se guardarán en el Servei durante 1 mes, después de la entrega de resultados.

The delivery time of results are no more than one week, except for emergency cases. Results can be delivered into a CD, USB, in paper or by electronic mailing and they are kept at the SG for one year after their delivery.

With each assay it is mandatory to submit a form of Assay Request and the Specific Invoice (UAB users or non-UAB users).

SGB is not responsible for the assay steps not performed by the SGB staff.

The use of self-service equipment requices training and authorization by the SGB and must be accompanied by a request form proved by the Principal Investigatior.

Sample	Technique		Minimal volume	Optimal concentration	Conditions Temp	Format	kit (assay appl.)	Results
DNA	Purification DNA		10µl	100ng/µl up to 10µg/µl	RT	Tube 0,2 ml		DNA in water
	Quantification Nanodrop		2µl	2ng/µl up to 3700ng/µl	RT	Tube 0,2 ml		Excel
	Quantification Qubit	dsDNA HS Assay	1-20µl	0,2-100ng	RT	Tube 0,2 ml		Excel
		dsDNA BR Assay	1-20µl	2-1000ng	RT	Tube 0,2 ml		Excel
		ssDNA Assay	1-20µl	1-200ng	RT	Tube 0,2 ml		Excel
		RNA Assay	1-20µl	5-100ng	-20ºC	Tube 0,2 ml		Excel
		rNA BR Assay	1-20µl	20-1000ng	-20ºC	Tube 0,2 ml		Excel
		Protein Assay	1-20µl	0,25-5ng	-20ºC	Tube 0,2 ml		Excel
	Sequencing ABI 3130		DNA 5µl	100 ng/µl	RT	Tube 0,2 ml or plate 96		File .abi File .seq
	Sequencing ABI 3130		Primer 2µl	5 µM	RT	Tube 0,2 ml or plate 96		File .abi File .seq
	High Throughput Sequencing		Ask to SGB	Ask to SGB				File .fastq
	Resuspension		---	---	RT	Tube 0,2 ml or plate 96	Indicate version Big Dye used	File .abi File .seq
	Gene Mapper		2µl	---	RT	Tube 0,2 ml or plate 96	Indicate filter	File .fsa prints
	Quality LAb Chip DNA		2µl	0,5ng/µl up to 50ng/µl	RT	Tube 0,2 ml		pdf
RNA	Quantification Nanodrop		2µl	2ng/µl up to 3700ng/µl	-20ºC	Tube 0,2 ml		excel
RNA	LAb Chip RNA		2µl	25-500ng/µl	-20ºC	Tube 0,2 ml		pdf
Microarrays	Printing Microgrid		20µl	100ng/µl	-20ºC	Plate384		Chips
	Labelling		10µl	1µg/µl	-20ºC	Tube 0,2 ml
	Hybridization Array Booster		---	---	-20ºC
	Scanning scan array		---	---	RT			Files.tiff
	Data generation Gene pix		---	---				Files.gpr