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EQUIPMENT |
MiSeq |
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KIT |
It depends on the sample to be sequenced. |
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SAMPLES |
Up to 96 samples/run. It is possible to mix samples from different species and the library prepared using different applications (i.e., de novo, small RNA and 16S metagenomics), in case the read length is the same. |
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CAPACITY |
Up to 96 samples sequencing 250 (soon 300) bases per chain and fragment from each sample's library. This yields up to 30 million reads and 7 Gb sequence. |
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RESULTS |
They must be recovered using a USB memory. |
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ACTIVITY |
CONDITIONS |
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Library preparation |
The material must diluted in water and to be into a 1.5 ml eppendorf tube. |
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Sample's library loading into the cartridge |
Ask the SGB. |
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Sequencing using customer's cartridge |
Ask the SGB. |