1) Creation of the microarray using a robot that prints the DNA probes |
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DEVICE |
ACTIVITY |
CONDITIONS |
MicroGrid II (BioRobotics) |
Manufacture of microarrays |
Spotting up to 100 slides simultaneously. |
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Generation of microarrays that contains the genome of the yeast S. cerevisiae |
The SGB routinely generates microarrays that contain the whole genome of the yeast S. cerevisiae. Probes are double-strain DNA fragments, onto a derivatized-amino support (UltraGaps, Corning). |
Thermocycler,PCR |
Synthesis and labelling of cDNAs |
The SGB can carry out synthesis reactions and labelling of cDNAs from RNA samples provided by the user. |
NANODROP |
Quality control of RNA |
Measurement of concentration and quality of RNA |
ArrayBooster (Advalytix). |
Hybridization of microarrays |
The user provides the microarrays (dimensions of a standard slide) |
ScanArray 4000 and ScanArray Gx Plus (Perkin Elmer) |
Scanning of microarrays |
Scanner equipped with two lasers and a specific software for the generation of data (ScanArray) |
(QuantArray |
Generation of data |
We offer the possibility of generating data using two different softwares (QuantArray/ScanExpress and GenePix). Results are an Excel file and the images of the microarrays. |