Tokuyasu method in cell cultures and tissues with criotalls
Inmunolocalization antigens in cell cultures and tissues. The samples are prepared using a mixture of chemical methods (fixation and cryoprotection) and physical methods (fixed in liquid nitrogen or propane). Subsequently, ultrathin cuts are made in cold conditions (-120ºC) on which the immunolocalization procedure (incubation in gold-labeled primary and secondary antibodies will take place in preparation for TEM observation.
Microscope used: JEOL JEM-1400
Immunolocalizations images with hydrophilic resins
Antigen immunolocalization of citoplasm and nucleus, as well as surface antigen immunolocalization of cell cultures and tissues. The samples are prepared using a mixture of chemical methods (fixation, cryoprotection, embedding in Lowicryl resin and UV) and physical methods(liquid nitrogen or propane fixation). Subsequently, ultrathin cuts are made at room temperature on which the immulocalization procedure (incubation in gold-labeled primary and secondary antibodies) will take place in preparation for TEM observation.
Microscope used: JEOL JEM-1400
