Estudios de dinámica de proteinas
Serial FRAP images of tubulin-GFP in a control and colcemid-treated cells. Typical recovery curves of the FRAP measurements (n=15-20) in non-treated and colcemid treated cells. Region of interest indicates the area that was photobleached and subsequently monitored for fluorescence recovery. Each panel in the FRAP series shows before (Pre-bleach) and immediately after photobleaching (1, 8, 30 90 and 260 seconds).
Microscopio utilizado: Leica SP5